Market Summary

The in situ hybridization (ISH) market is experiencing accelerated growth as precision diagnostics and molecular pathology continue to advance. ISH, a powerful technique used to detect specific nucleic acid sequences directly in tissue samples, is becoming indispensable in oncology, infectious disease detection, and neurological research. This blog provides a comprehensive overview of the global ISH market, including trends, segmentation, top players, regional performance, innovations, and more.

In situ hybridization (ISH) is a molecular technique that allows scientists to detect specific DNA or RNA sequences directly in tissue samples or cells. It is widely used in pathology, genetic diagnostics, neuroscience, and oncology.

Market Size & Growth

• Global Market Value (2021): Approx. $1523.4 million

• Projected Market Value (2028): Approx. $2.64 billion

• Expected CAGR (2021–2028): Approx. 9%

• Key Drivers:

  • Increased prevalence of cancer and genetic disorders

  • Advancements in spatial genomics and digital pathology

  • Expansion of companion diagnostics in personalized medicine

  • Growth in infectious disease research and neurological applications

What is In Situ Hybridization (ISH)?

ISH is a molecular technique that uses labeled complementary DNA or RNA probes to detect specific genetic material in fixed tissues or cells. It enables localization of gene expression, chromosomal abnormalities, and viral DNA/RNA within the sample.

Would you like me to add more details on specific companies or market trends? Let me know! CLICK HERE

Top Companies in the Global ISH Market (2025)

Bio-Techne Corporation (Advanced Cell Diagnostics)

• Headquarters: Minneapolis, Minnesota, USA

• Founded: 1976

• Employees: Approx. 3,000

• Revenue (2024): $1.2 billion

• Notable ISH Products: RNAscope®, BaseScope®

• Highlights: Leaders in RNA ISH and spatial biology, with superior signal amplification and detection technologies.

Abbott Laboratories

• Headquarters: Illinois, USA

• Founded: 1888

• Employees: Approx. 115,000

• Revenue (2024): $43 billion

• Notable ISH Products: Vysis® FISH assays

• Highlights: Pioneer in fluorescent in situ hybridization (FISH) for oncology and cytogenetics.

Agilent Technologies

• Headquarters: Santa Clara, California, USA

• Founded: 1999

• Employees: Approx. 18,000

• Revenue (2024): $7.1 billion

• Notable ISH Products: Dako FISH and CISH kits

• Highlights: Strong in oncology and companion diagnostics; robust integration with pathology workflows.

Leica Biosystems (Danaher Corporation)

• Headquarters: Illinois, USA

• Founded: 1989

• Employees: Approx. 2,000

• Revenue (2024): Approx. $750 million

• Notable ISH Products: BOND RX™ platform, FISH/CISH probes

• Highlights: Automates tissue diagnostics and ISH processing, leading in high-throughput systems.

Thermo Fisher Scientific

• Headquarters: Waltham, Massachusetts, USA

• Founded: 1956

• Employees: Approx. 125,000

• Revenue (2024): $45.8 billion

• Notable ISH Products: ViewRNA ISH Assays

• Highlights: Combines molecular biology expertise with spatial transcriptomics.

BioGenex Laboratories

• Headquarters: Fremont, California, USA

• Founded: 1981

• Employees: Approx. 200

• Revenue (2024): Approx. $60 million

• Notable ISH Products: Xmatrx® system, FISH/ISH probes

• Highlights: Pioneer in fully automated ISH workflows and IHC-ISH dual staining.

PerkinElmer Inc. (Now part of Revvity, Inc.)

• Headquarters: Waltham, Massachusetts, USA

• Founded: 1937

• Employees: Approx. 17,000

• Revenue (2024): Approx. $5.3 billion

• Notable ISH Products: ViewRNA assays, FISH kits

• Highlights: Expanding spatial biology capabilities for precision diagnostics.

Roche Diagnostics

• Headquarters: Basel, Switzerland

• Founded: 1896

• Employees: Approx. 100,000

• Revenue (2024): $63 billion

• Notable ISH Products: Ventana FISH and CISH

• Highlights: Leaders in automated staining and cancer diagnostics.

Regional Analysis

North America

• Largest market, driven by clinical adoption, cancer diagnostics, and tech advancements.

• Key countries: United States, Canada

Europe

• Growth fueled by rising R&D and precision medicine adoption.

• Key countries: Germany, UK, France

Asia-Pacific

• Fastest-growing region with increased investment in genomics and diagnostics.

• Key countries: China, Japan, India

Latin America & Middle East

• Emerging markets focusing on healthcare infrastructure and diagnostic accessibility.

Segmentation Analysis

By Technique

• Fluorescent In Situ Hybridization (FISH)

• Chromogenic In Situ Hybridization (CISH)

• RNA ISH

• DNA ISH

By Application

• Cancer Diagnostics

• Infectious Disease Detection

• Genetic Disorder Research

• Neuroscience & Developmental Biology

• Drug Discovery

By End User

• Hospitals & Diagnostic Labs

• Academic & Research Institutions

• Biotechnology & Pharmaceutical Companies

• Contract Research Organizations (CROs)

Market Trends and Growth Opportunities (2025)

• Spatial Genomics Expansion: Growing demand for single-cell and spatial resolution in tissue analysis.

• AI-Enhanced Pathology: Automated interpretation of ISH results using deep learning.

• Multiplexing Technologies: Simultaneous detection of multiple targets in a single assay.

• Companion Diagnostics: ISH plays a critical role in targeted therapies for oncology.

• Digital Pathology Integration: Remote viewing and analysis of hybridized slides.

Recent Innovations

• RNAscope HiPlex™: Multiplex RNA profiling in FFPE tissues

• Automated ISH platforms: Higher throughput and reduced human error

• CRISPR-assisted ISH: Enhanced specificity in gene detection

• 3D ISH Imaging: Visualizing gene expression in 3D tissue structures

Frequently Asked Questions (FAQs)

»  What is in situ hybridization used for?

ISH is used to detect and localize specific DNA or RNA sequences in cells and tissues, commonly applied in cancer diagnosis, neuroscience, and virology.

»  What is the difference between FISH and CISH?

FISH uses fluorescent probes, while CISH uses chromogenic labels that are visible under a standard light microscope.

»  Which companies lead the ISH market?

Bio-Techne (RNAscope), Abbott (Vysis FISH), Agilent (Dako), and Leica Biosystems are among the market leaders.

»  What makes RNA ISH important?

RNA ISH enables detection of gene expression patterns, playing a crucial role in precision medicine and biomarker discovery.

»  Is automation common in ISH?

Yes. Automated platforms are increasingly adopted for high-throughput processing and reduced operator variability.

The global in situ hybridization market in 2025 is marked by strong innovation, clinical relevance, and increasing integration into personalized medicine. The top companies featured here are advancing the field through automation, multiplexing, and spatial biology technologies, reinforcing ISH’s role as a cornerstone of modern molecular diagnostics.

About US:

IMIR Market Research is a global market intelligence and consulting organization that provides syndicated research reports, customized research reports, and consulting services. We are known for our actionable insights and authentic reports in various domains including, Semiconductor, aerospace, Automation, Agriculture, Food & Beverages, Automotive, Chemicals and Materials, and virtually all domains and an exhaustive list of sub-domains under the sun. We create value for clients through our highly reliable and accurate reports.

Contact Us: IMIR Market Research Pvt. Ltd.

Email: sales@intellectualmarketinsights.com

Call Us: +1 (814) 487 8486